US 11,885,947 B2
Lattice light sheet microscope and method for tiling lattice light sheet in lattice light sheet microscope
Liang Gao, Zhejiang (CN); and Bi-Chang Chen, Taiwan (CN)
Assigned to Westlake University, Zhejiang (CN)
Appl. No. 17/289,554
Filed by WESTLAKE UNIVERSITY, Zhejiang (CN)
PCT Filed Jul. 23, 2019, PCT No. PCT/CN2019/097237
§ 371(c)(1), (2) Date Apr. 28, 2021,
PCT Pub. No. WO2020/087998, PCT Pub. Date May 7, 2020.
Claims priority of provisional application 62/751,635, filed on Oct. 28, 2018.
Prior Publication US 2022/0019066 A1, Jan. 20, 2022
Int. Cl. G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/14 (2006.01)
CPC G02B 21/0032 (2013.01) [G01N 21/6458 (2013.01); G02B 21/0048 (2013.01); G02B 21/0076 (2013.01); G02B 21/14 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A lattice light sheet microscope which comprises a spatial light modulator, an excitation objective, a transparent annular diaphragm, and a first galvanometer, wherein
an optical modulation plane of the spatial light modulator is conjugated to an image plane of the excitation objective, and the spatial light modulator is configured to modulate illumination light;
the transparent annular diaphragm is arranged at a plane that is conjugated to an entrance pupil of the excitation objective to filter illumination light modulated by the spatial light modulator;
the spatial light modulator is a single spatial light modulator, and is configured to: generate an optical lattice by loading a phase map obtained from a center section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading phase map obtained from an off-center section of the corresponding optical lattice; and
the first galvanometer is configured to scan each optical lattice in an extending direction of the optical lattice to form a tiled lattice light sheet.