CPC G01N 33/6872 (2013.01) [C12N 15/1065 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6841 (2013.01); C12Q 2522/10 (2013.01)] | 32 Claims |
1. A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell or nucleus, comprising:
contacting a permeabilized cell or nucleus immobilized on a solid surface with a first specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the first specific binding agent is coupled to a nuclease in an inactive state;
activating the nuclease and cleaving DNA bound to the chromatin-associated factor of interest to thereby excise the DNA bound to the chromatin-associated factor of interest and release it to a supernatant;
isolating the excised DNA in the supernatant; and
determining the sequence of the excised DNA, thereby mapping binding of a chromatin-associated factor of interest to a sequence of DNA in the cell or nucleus.
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