US 11,885,810 B2
Detection of membrane proteins by mass spectrometry
Dror S. Chorev, Oxford (GB); and Carol V. Robinson, Oxford (GB)
Assigned to OXFORD UNIVERSITY INNOVATION LIMITED, Oxford (GB)
Appl. No. 17/272,591
Filed by OXFORD UNIVERSITY INNOVATION LIMITED, Oxford (GB)
PCT Filed Aug. 30, 2019, PCT No. PCT/GB2019/052421
§ 371(c)(1), (2) Date Mar. 1, 2021,
PCT Pub. No. WO2020/049274, PCT Pub. Date Mar. 12, 2020.
Claims priority of application No. 1814454 (GB), filed on Sep. 5, 2018.
Prior Publication US 2021/0325402 A1, Oct. 21, 2021
Int. Cl. G01N 33/68 (2006.01); H01J 49/16 (2006.01)
CPC G01N 33/6848 (2013.01) [H01J 49/165 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method of detecting a membrane protein by mass spectrometry, wherein the method comprises:
(a) providing a solution comprising a native membrane vesicle having a lipid bilayer to which said membrane protein is attached;
(b) sonicating the vesicle in the presence of a mass spectrometry-compatible buffer;
(c) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector;
(d) vaporising the sonicated solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the vesicle;
(e) ionising the membrane protein;
(f) resolving the ionised membrane protein using the mass analyser; and
(g) detecting the resolved membrane protein using the detector.