US 11,885,803 B2
Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
Daniel Takashi Kamei, Monterey Park, CA (US); Yin To Chiu, Irvine, CA (US); Benjamin Ming Wu, San Marino, CA (US); and Garrett L. Mosley, Newport Beach, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Feb. 16, 2022, as Appl. No. 17/673,530.
Application 17/673,530 is a division of application No. 15/756,542, granted, now 11,287,426, previously published as PCT/US2016/050257, filed on Sep. 2, 2016.
Claims priority of provisional application 62/214,801, filed on Sep. 4, 2015.
Prior Publication US 2022/0252598 A1, Aug. 11, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/569 (2006.01); G01N 33/543 (2006.01); G01N 33/483 (2006.01); G01N 33/49 (2006.01); G01N 33/493 (2006.01)
CPC G01N 33/56927 (2013.01) [G01N 33/4833 (2013.01); G01N 33/49 (2013.01); G01N 33/493 (2013.01); G01N 33/54388 (2021.08); G01N 33/54391 (2021.08); G01N 33/569 (2013.01); G01N 33/56944 (2013.01); G01N 33/56961 (2013.01); G01N 33/56983 (2013.01)] 19 Claims
 
1. A method of concentrating an analyte using an aqueous two-phase system, said method comprising:
providing a porous matrix comprising salt-polymer aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution as said ATPS moves through said porous matrix wherein said ATPS is provided as a dehydrated salt and a dehydrated polymer that when rehydrated form said mixed phase of an aqueous two-phase system, where said dehydrated salt is disposed in said porous matrix upstream from said dehydrated polymer;
applying a sample solution comprising said analyte to said porous matrix where said sample solution rehydrates the dehydrated salt and dehydrated polymer to form the mixed phase solution that separates into a first phase solution and a second phase solution as said ATPS moves through said porous matrix and concentrates the analyte into the first phase solution or the second phase solution or an interface between the first phase solution and second phase solution.