US 11,884,986 B2
Transgenic corn event MON87403 and methods for detection thereof
Thomas R. Adams, Stonington, CT (US); John A. Korte, Westerly, RI (US); Anagha M. Sant, St. Louis, MO (US); and J. Philip Taylor, Saint Peters, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Aug. 15, 2022, as Appl. No. 17/819,853.
Application 17/819,853 is a division of application No. 17/075,591, filed on Oct. 20, 2020, granted, now 11,466,330.
Application 17/075,591 is a division of application No. 16/516,008, filed on Jul. 18, 2019, granted, now 10,858,710, issued on Dec. 8, 2020.
Application 16/516,008 is a division of application No. 16/160,841, filed on Oct. 15, 2018, granted, now 10,407,741, issued on Sep. 10, 2019.
Application 16/160,841 is a division of application No. 15/289,635, filed on Oct. 10, 2016, granted, now 10,233,507, issued on Mar. 19, 2019.
Application 15/289,635 is a division of application No. 14/507,734, filed on Oct. 6, 2014, granted, now 9,469,880, issued on Oct. 18, 2016.
Claims priority of provisional application 61/888,978, filed on Oct. 9, 2013.
Prior Publication US 2023/0056124 A1, Feb. 23, 2023
Int. Cl. C12N 15/82 (2006.01); C12Q 1/6895 (2018.01); C07K 14/415 (2006.01); A23L 7/10 (2016.01)
CPC C12Q 1/6895 (2013.01) [A23L 7/10 (2016.08); C07K 14/415 (2013.01); C12N 15/8261 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); Y02A 40/146 (2018.01)] 6 Claims
 
1. A method of determining the zygosity of a corn plant genome comprising corn event MON87403 DNA in a sample comprising:
(a) contacting the sample with a first pair of DNA molecules and a second distinct pair of DNA molecules that:
(i) when used together in a nucleic acid amplification reaction with corn event MON87403 DNA, produces a first amplicon that is diagnostic for corn event MON87403; and
(ii) when used together in a nucleic acid amplification reaction with corn genomic DNA other than corn event MON87403 DNA, produces a second amplicon that is diagnostic for corn wild type genomic DNA other than corn event MON87403 DNA;
(b) performing a nucleic acid amplification reaction; and
(c) detecting said first amplicon and said second amplicon;
wherein the first amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, and
wherein the presence of said first and second amplicons is diagnostic of a heterozygous genome in said sample, and wherein the presence of only said first amplicon is diagnostic of a genome homozygous for corn event MON87403 in said sample.