US 11,884,983 B2
Method for predicting resistance
Nina Santi, Trondheim (NO); Thomas Moen, Ås (NO); and Jørgen Ødegård, Sande I Vestfold (NO)
Assigned to Aqua Gen AS, Trondheim (NO)
Appl. No. 15/527,592
Filed by Aqua Gen AS, Trondheim (NO)
PCT Filed Nov. 18, 2015, PCT No. PCT/NO2015/050218
§ 371(c)(1), (2) Date May 17, 2017,
PCT Pub. No. WO2016/080844, PCT Pub. Date May 26, 2016.
Claims priority of application No. 20141382 (NO), filed on Nov. 18, 2014.
Prior Publication US 2019/0241980 A1, Aug. 8, 2019
Int. Cl. C12Q 1/6888 (2018.01)
CPC C12Q 1/6888 (2013.01) [C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01)] 10 Claims
 
1. A method for obtaining rainbow trout having increased resistance to infectious pancreatic necrosis (IPN), said method comprising:
a) providing an initial population of rainbow trout;
b) obtaining a nucleic acid sample from at least one individual rainbow trout within said initial population;
c) detecting in each of said nucleic acid samples the presence of at least one allele conferring IPN resistance (“IPN resistance allele”) within the genome of said rainbow trout, wherein the at least one IPN resistance allele is an allele of at least one single nucleotide polymorphism (SNP) selected from the SNPs listed in the following table:
 
 
 
 
 
 Position in
 
 IPN Non-IPN
SNP Name SEQ ID GenBank GenBank dbSNP ss- resistance resistance
# Affymetrix ID NO: Contig Contig No (SS#) allele allele
 
1 AX-89929954 1 FR904293.1 1651243 1398298005 C A
2 AX-89918280 2 FR904293.1 1353665 1399389616 G A
4 AX-89960828 4 FR932837.1   3160 1399779599 T   C ;
 
d) selecting a rainbow trout from said initial population based on the presence of the at least one IPN resistance allele in the nucleic acid samples; and
e) mating said selected rainbow trout with a second rainbow trout to produce progeny rainbow trout comprising increased resistance to IPN.