US 11,884,982 B2
Tumor marker, methylation detection reagent, kit and use thereof
Xianglin Liu, Guangzhou (CN); Rongsong Zhao, Guangzhou (CN); and Hongzhi Zou, Guangzhou (CN)
Assigned to CREATIVE BIOSCIENCES (GUANGZHOU) CO., Guangdong (CN)
Appl. No. 17/057,410
Filed by CREATIVE BIOSCIENCES (GUANGZHOU) CO., LTD., Guangdong (CN)
PCT Filed May 5, 2019, PCT No. PCT/CN2019/085583
§ 371(c)(1), (2) Date Nov. 20, 2020,
PCT Pub. No. WO2019/223516, PCT Pub. Date Nov. 28, 2019.
Claims priority of application No. 201810494478.2 (CN), filed on May 22, 2018.
Prior Publication US 2021/0207222 A1, Jul. 8, 2021
Int. Cl. C12Q 1/6886 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 1/6806 (2013.01); C12Q 2600/154 (2013.01)] 14 Claims
 
1. A methylation detection kit of COL4A1 gene, comprising:
a gene capturing reagent comprising a magnetic bead probe complex;
a capture sequence having a nucleotide sequence having at least 85% identity to SEQ ID NO: 1;
a first primer having a nucleotide sequence having at least 85% identity to SEQ ID NO: 2;
a second primer having a nucleotide sequence having at least 85% identity to SEQ ID NO: 3; and
a probe having a nucleotide sequence having at least 85% identity to SEQ ID NO: 4.