US 11,884,975 B2
Sequencing methods and compositions for prenatal diagnoses
Richard P. Rava, Redwood City, CA (US); Manjula Chinnappa, Foster City, CA (US); David A. Comstock, Sunnyvale, CA (US); Gabrielle Heilek, Mountain View, CA (US); and Brian Kent Rhees, Chandler, AZ (US)
Assigned to VERINATA HEALTH, INC., Redwood City, CA (US)
Filed by Verinata Health, Inc., Redwood City, CA (US)
Filed on Mar. 5, 2021, as Appl. No. 17/193,279.
Application 17/193,279 is a continuation of application No. 15/601,951, filed on May 22, 2017, granted, now 10,941,442.
Application 15/601,951 is a continuation of application No. 12/958,353, filed on Dec. 1, 2010, granted, now 9,657,342, issued on May 23, 2017.
Claims priority of provisional application 61/455,849, filed on Oct. 26, 2010.
Claims priority of provisional application 61/407,017, filed on Oct. 26, 2010.
Claims priority of provisional application 61/360,837, filed on Jul. 1, 2010.
Claims priority of provisional application 61/296,358, filed on Jan. 19, 2010.
Prior Publication US 2021/0340613 A1, Nov. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); G16B 30/10 (2019.01); G16B 20/10 (2019.01); G16H 10/40 (2018.01); C12Q 1/6883 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); G16B 20/10 (2019.02); G16B 30/10 (2019.02); G16H 10/40 (2018.01); C12Q 1/6883 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/112 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for preparing a sequencing library from a test sample comprising nucleic acid molecules, wherein said nucleic acids molecules are human cell-free DNA (cfDNA) molecules, wherein the method comprises the consecutive steps of end-repairing, dA-tailing and adaptor ligating said nucleic acids molecules, wherein said consecutive steps exclude purifying the end-repaired products prior to the dA-tailing step and exclude purifying the dA-tailing products prior to the adaptor-ligating step, and wherein said consecutive steps are performed in less than 1 hour.