CPC C12Q 1/6806 (2013.01) [C12Q 1/6811 (2013.01); C12Q 1/6834 (2013.01); C40B 20/04 (2013.01); C40B 50/16 (2013.01); C40B 50/18 (2013.01); G06K 19/06093 (2013.01)] | 18 Claims |
1. A method for generating nucleic acid barcode molecules, comprising:
(a) providing a plurality of nucleic acid molecules coupled to a support;
(b) coupling one or more additional nucleic acid molecules to nucleic acid molecules of the plurality of nucleic acid molecules coupled to the support, thereby assembling a plurality of nucleic acid barcode molecules coupled to the support, wherein the plurality of nucleic acid barcode molecules comprises a plurality of target products and a plurality of off-target products; and
(c) digesting off-target products of the plurality of off-target products with an exonuclease, wherein target products of the plurality of target products comprise an exonuclease-resistant bond, and wherein fewer than 50% of the target products comprising the exonuclease-resistant bond are digested with the exonuclease.
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