US 11,884,959 B2
Methods and systems for automated assessment of antibiotic sensitivity
Raphael Rodolphe Marcelpoil, Corenc (FR)
Assigned to BD KIESTRA B.V., Drachten (NL)
Filed by BD KIESTRA B.V., Drachten (NL)
Filed on Mar. 18, 2022, as Appl. No. 17/698,518.
Application 17/698,518 is a continuation of application No. 16/650,087, granted, now 11,319,575, previously published as PCT/EP2018/076257, filed on Sep. 27, 2018.
Claims priority of provisional application 62/564,727, filed on Sep. 28, 2017.
Prior Publication US 2022/0213523 A1, Jul. 7, 2022
Int. Cl. C12Q 1/18 (2006.01); C12Q 1/20 (2006.01); G06T 7/11 (2017.01); G01N 21/17 (2006.01); G06T 7/00 (2017.01); G06T 7/90 (2017.01); G06V 20/69 (2022.01)
CPC C12Q 1/18 (2013.01) [C12Q 1/20 (2013.01); G01N 21/17 (2013.01); G06T 7/0016 (2013.01); G06T 7/11 (2017.01); G06T 7/90 (2017.01); G06V 20/69 (2022.01); G06V 20/693 (2022.01); G06V 20/695 (2022.01)] 24 Claims
OG exemplary drawing
 
1. A method in a processor for antibiotic susceptibility testing comprising:
providing a culture plate inoculated with a biological sample, the culture plate having culture media and at least one antibiotic disk disposed thereon;
receiving first and second image data of the culture plate generated with an image sensor and wherein the first image data serves as a pre-growth reference, the first image data and second image data respectively representing first and second captured images of the culture plate having a plurality of antibiotic disks thereon, the first and second captured images taken at separate times;
identifying a no-growth region adjacent the antibiotic disk and a growth region further removed from the antibiotic disk relative to no-growth region;
obtaining a polar transform of contrast information obtained from a comparison of the first and second captured images whereby an image of the antibiotic disk is converted into a first light band having a first intensity, an image of the no-growth region is converted to a second light band having a second light intensity; and an image of the growth region is converted to a third light band having a third light intensity wherein the second intensity is a lower than the first intensity and the third intensity is greater than the second intensity but less than the first intensity;
forming a distance map from intensity values in the first, second, and third light bands; and
determining a minimum inhibitory distance from the distance map.