CPC C12Q 1/18 (2013.01) [C12Q 1/20 (2013.01); G01N 21/17 (2013.01); G06T 7/0016 (2013.01); G06T 7/11 (2017.01); G06T 7/90 (2017.01); G06V 20/69 (2022.01); G06V 20/693 (2022.01); G06V 20/695 (2022.01)] | 24 Claims |
1. A method in a processor for antibiotic susceptibility testing comprising:
providing a culture plate inoculated with a biological sample, the culture plate having culture media and at least one antibiotic disk disposed thereon;
receiving first and second image data of the culture plate generated with an image sensor and wherein the first image data serves as a pre-growth reference, the first image data and second image data respectively representing first and second captured images of the culture plate having a plurality of antibiotic disks thereon, the first and second captured images taken at separate times;
identifying a no-growth region adjacent the antibiotic disk and a growth region further removed from the antibiotic disk relative to no-growth region;
obtaining a polar transform of contrast information obtained from a comparison of the first and second captured images whereby an image of the antibiotic disk is converted into a first light band having a first intensity, an image of the no-growth region is converted to a second light band having a second light intensity; and an image of the growth region is converted to a third light band having a third light intensity wherein the second intensity is a lower than the first intensity and the third intensity is greater than the second intensity but less than the first intensity;
forming a distance map from intensity values in the first, second, and third light bands; and
determining a minimum inhibitory distance from the distance map.
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