US 11,884,951 B2
Method for producing RNA
Shuhei Hashiro, Kanagawa (JP); Hisashi Yasueda, Kanagawa (JP); Mayu Mitsuhashi, Kanagawa (JP); Sergei Vladimirovich Mashko, Moscow (RU); Aleksandr Aleksandrovich Krylov, Moscow (RU); and Yuliya Sergeevna Lobanova, Moscow (RU)
Assigned to AJINOMOTO CO., INC., Tokyo (JP)
Filed by AJINOMOTO CO., INC., Tokyo (JP)
Filed on May 21, 2021, as Appl. No. 17/326,677.
Application 17/326,677 is a continuation of application No. 16/582,385, filed on Sep. 25, 2019, granted, now 11,046,986.
Application 16/582,385 is a continuation of application No. PCT/JP2018/003692, filed on Feb. 2, 2018.
Claims priority of application No. 2017-063777 (JP), filed on Mar. 28, 2017; and application No. RU2017129988 (RU), filed on Aug. 24, 2017.
Prior Publication US 2021/0269840 A1, Sep. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12N 15/77 (2006.01); C12N 1/20 (2006.01); C12R 1/13 (2006.01); C12R 1/05 (2006.01)
CPC C12P 19/34 (2013.01) [C12N 1/205 (2021.05); C12N 15/1003 (2013.01); C12N 15/77 (2013.01); C12R 2001/05 (2021.05); C12R 2001/13 (2021.05)] 10 Claims
 
1. A method for producing objective RNA, the method comprising:
A) culturing a coryneform bacterium having an expression unit for the objective RNA in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium; and
B) collecting the objective RNA from the cells,
wherein the bacterium has been modified so that the activity of ribonuclease III is reduced as compared with a non-modified strain by attenuating the expression of a gene encoding ribonuclease III;
wherein the ribonuclease III is a protein selected from the group consisting of:
(a) a protein comprising the amino acid sequence of SEQ ID NO: 52;
(b) a protein comprising the amino acid sequence of SEQ ID NO: 52, but which includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and having ribonuclease III activity; and
(c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO: 52, and having ribonuclease III activity.