US 11,884,941 B2
Non-canonical lipoproteins with programmable assembly and architecture and method of making non-canonical lipoproteins
Davoud Mozhdehi, Syracuse, NY (US)
Assigned to SYRACUSE UNIVERSITY, Syracuse, NY (US)
Filed by Davoud Mozhdehi, Syracuse, NY (US)
Filed on Nov. 18, 2021, as Appl. No. 17/529,860.
Claims priority of provisional application 63/115,696, filed on Nov. 19, 2020.
Prior Publication US 2022/0154151 A1, May 19, 2022
Int. Cl. C12P 7/64 (2022.01); C12N 9/10 (2006.01)
CPC C12N 9/1029 (2013.01) [C12P 7/64 (2013.01); C12Y 203/01097 (2013.01)] 8 Claims
 
1. A method of synthesizing a non-naturally occurring lipoprotein, comprising the steps of:
modifying a host organism to express a Saccharomyces cerevisiae N-myristoyl transferase;
modifying the host organism to express an intrisincally disordered peptide polymer fused to a peptide substrate of the N-myristoyl transferase; and
coexpressing the N-myristoyl transferase and the intrinsically disordered peptide polymer from the host organism in the presence of an expression media having a non-natural fatty acid having the formula R—COOH, where R contains a linear chain of no more than 15 atoms of C, N, O or S, so that the intrinsically disordered peptide polymer fused to the peptide substrate is subjected to post-translational modification by the host organism to form a non-naturally occurring lipoprotein.