CPC C12N 9/1029 (2013.01) [C12P 7/64 (2013.01); C12Y 203/01097 (2013.01)] | 8 Claims |
1. A method of synthesizing a non-naturally occurring lipoprotein, comprising the steps of:
modifying a host organism to express a Saccharomyces cerevisiae N-myristoyl transferase;
modifying the host organism to express an intrisincally disordered peptide polymer fused to a peptide substrate of the N-myristoyl transferase; and
coexpressing the N-myristoyl transferase and the intrinsically disordered peptide polymer from the host organism in the presence of an expression media having a non-natural fatty acid having the formula R—COOH, where R contains a linear chain of no more than 15 atoms of C, N, O or S, so that the intrinsically disordered peptide polymer fused to the peptide substrate is subjected to post-translational modification by the host organism to form a non-naturally occurring lipoprotein.
|