US 11,884,923 B2
Fructose-4-epimerase and method of producing tagatose using the same
Young Mi Lee, Seoul (KR); Eul-Soo Park, Seoul (KR); Il Hyang Park, Seoul (KR); Sun Mi Shin, Seoul (KR); Sung Jae Yang, Seoul (KR); Ran Young Yoon, Seoul (KR); Eun Jung Choi, Seoul (KR); Seong Bo Kim, Seoul (KR); and Seung Won Park, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/252,706
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Sep. 27, 2019, PCT No. PCT/KR2019/012621
§ 371(c)(1), (2) Date Dec. 15, 2020,
PCT Pub. No. WO2020/067788, PCT Pub. Date Apr. 2, 2020.
Claims priority of application No. 10-2018-0116609 (KR), filed on Sep. 28, 2018; application No. 10-2018-0117237 (KR), filed on Oct. 1, 2018; and application No. 10-2019-0099828 (KR), filed on Aug. 14, 2019.
Prior Publication US 2021/0363533 A1, Nov. 25, 2021
Int. Cl. C12N 15/52 (2006.01); C12N 9/88 (2006.01); C12N 15/70 (2006.01); C12P 19/02 (2006.01)
CPC C12N 15/52 (2013.01) [C12N 9/88 (2013.01); C12N 15/70 (2013.01); C12P 19/02 (2013.01); C12Y 401/0204 (2013.01)] 8 Claims
 
1. A fructose-4-epimerase variant having i) substitution of another amino acid for one or more amino acids selected from the group consisting of amino acids at positions 52, 136, 197, 317, and 320, or substitution of glutamic acid (E) for an amino acid at position 414 from the N-terminus of fructose-4-epimerase comprising the amino acid sequence of SEQ ID NO: 1, wherein the variant comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1.