US 11,884,918 B2
Systems and methods for modulating CRISPR activity
Reed Kelso, San Francisco, CA (US); Jared Carlson-Stevermer, Burlingame, CA (US); Sahil Joshi, Somerville, MA (US); Travis Maures, La Jolla, CA (US); Anastasia Kadina, Sunnyvale, CA (US); and John Andrew Walker, II, San Leandro, CA (US)
Assigned to Synthego Corporation, Redwood City, CA (US)
Filed by Synthego Corporation, Redwood City, CA (US)
Filed on Jul. 23, 2021, as Appl. No. 17/384,328.
Application 17/384,328 is a continuation of application No. PCT/US2020/015127, filed on Jan. 25, 2020.
Claims priority of provisional application 62/939,553, filed on Nov. 22, 2019.
Claims priority of provisional application 62/876,177, filed on Jul. 19, 2019.
Claims priority of provisional application 62/797,122, filed on Jan. 25, 2019.
Prior Publication US 2022/0073912 A1, Mar. 10, 2022
Int. Cl. C12N 15/113 (2010.01); C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01)
CPC C12N 15/111 (2013.01) [C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 2310/20 (2017.05)] 9 Claims
 
1. A CRISPR OFF polynucleotide comprising:
(a) a guide sequence configured to anneal to a target sequence in a target nucleic acid molecule;
(b) a sequence configured to bind to a CRISPR enzyme; and
(c) a cleavable linker positioned at position 57 and/or 74 in the sequence configured to bind to the CRISPR enzyme wherein position 1 is at a 5′ end of the guide sequence and positions are counted from 5′ to 3′, and wherein the CRISPR OFF polynucleotide is inactivated when the cleavable linker is cleaved.