	US 11,884,916 B2
	Materials and methods for the synthesis of error-minimized nucleic acid molecules
Daniel G Gibson, Carlsbad, CA (US); Nicky Caiazza, Rancho Santa Fe, CA (US); and Toby H. Richardson, San Diego, CA (US)
Assigned to Telesis Bio Inc., La Jolla, CA (US)
Filed by Codex DNA, Inc., San Diego, CA (US)
Filed on Jul. 2, 2020, as Appl. No. 16/920,204.
Application 15/667,515 is a division of application No. 13/757,633, filed on Feb. 1, 2013, granted, now 9,771,576, issued on Sep. 26, 2017.
Application 16/920,204 is a continuation of application No. 15/667,515, filed on Aug. 2, 2017, granted, now 10,704,041.
Claims priority of provisional application 61/593,813, filed on Feb. 1, 2012.
Prior Publication US 2020/0332286 A1, Oct. 22, 2020
Int. Cl. C12N 15/10 (2006.01); C12N 9/22 (2006.01); C12Q 1/6844 (2018.01); C12P 19/34 (2006.01)

CPC C12N 15/1093 (2013.01) [C12N 9/22 (2013.01); C12P 19/34 (2013.01); C12Q 1/6844 (2013.01); C12Y 301/11 (2013.01); C12Y 301/21 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/036 (2013.01)]

12 Claims

1. A composition comprising: (i) a molecule having a unidirectional mismatch endonuclease activity having at least 80% sequence identity to a sequence selected from the group consisting of: SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 28, and SEQ ID NO: 29, and (ii) a molecule having unidirectional exonuclease activity of the same directionality as the unidirectional mismatch endonuclease activity of (i).