US 11,884,911 B2
Cell culture device, system and methods of use thereof
Christopher Mason, Cambridge, MA (US); and Farlan Veraitch, London (GB)
Assigned to Oribiotech Ltd., London (GB)
Filed by ORIBiotech Ltd, London (GB)
Filed on Dec. 18, 2020, as Appl. No. 17/127,255.
Application 17/127,255 is a continuation of application No. 15/575,105, granted, now 11,208,626, previously published as PCT/GB2016/051451, filed on May 20, 2016.
Claims priority of application No. 1508752 (GB), filed on May 21, 2015.
Prior Publication US 2021/0130765 A1, May 6, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); A61M 5/28 (2006.01); C12M 1/00 (2006.01); A61M 5/148 (2006.01)
CPC C12M 41/44 (2013.01) [A61M 5/148 (2013.01); A61M 5/282 (2013.01); C12M 23/26 (2013.01); C12M 41/32 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container, the method further comprising the steps of:
(i) genetic modification; and
(ii) cytokine stimulation; and
(iii) expansion,
wherein each step of the method is carried out within the internal lumen of the container without the cells leaving the internal lumen of the container.