	US 11,884,741 B2
	Method for improving thermal stability of antibody and method for producing modified antibody
Tomofumi Nakada, Kobe (JP); and Nobuyuki Ide, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Feb. 22, 2021, as Appl. No. 17/181,068.
Claims priority of application No. 2020-029711 (JP), filed on Feb. 25, 2020.
Prior Publication US 2021/0347910 A1, Nov. 11, 2021
Int. Cl. C07K 16/32 (2006.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01); C07K 1/22 (2006.01); C07K 1/34 (2006.01)

CPC C07K 16/32 (2013.01) [C07K 16/005 (2013.01); C07K 16/2827 (2013.01); C07K 1/22 (2013.01); C07K 1/34 (2013.01); C07K 2317/24 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01); C07K 2317/94 (2013.01)]

17 Claims

1. A method for improving thermal stability of an antibody, comprising:

in a heavy chain of the antibody, changing at least one amino acid residue selected from the group consisting of 8th to 11th amino acid residues based on Kabat method to a cysteine residue, and changing at least one amino acid residue selected from the group consisting of 109th and 110th amino acid residues based on IMGT method to a cysteine residue,

wherein the modified antibody has improved thermal stability as compared to the unmodified antibody; and

wherein the modified antibody is Fab, Fab′, F(ab′)2, reduced IgG, IgG, IgA, IgM, IgD or IgE.