	US 11,884,738 B2
	Methods and compositions for activation of T cells using nanoparticles conjugated with multiple ligands for binding receptors on T cells
Andrew Wang, Durham, NC (US); and Yu Mi, Chapel Hill, NC (US)
Assigned to The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed by The University of North Carolina at Chapel Hill, Chapel Hill, NC (US)
Filed on Mar. 28, 2018, as Appl. No. 15/938,999.
Claims priority of provisional application 62/479,665, filed on Mar. 31, 2017.
Prior Publication US 2018/0282423 A1, Oct. 4, 2018
Int. Cl. A61K 47/50 (2017.01); C07K 16/28 (2006.01); A61K 39/395 (2006.01); A61K 45/06 (2006.01); C07K 16/30 (2006.01); A61K 47/69 (2017.01); A61P 35/00 (2006.01); A61K 39/00 (2006.01)

CPC C07K 16/2878 (2013.01) [A61K 39/3955 (2013.01); A61K 45/06 (2013.01); A61K 47/6935 (2017.08); A61K 47/6937 (2017.08); A61P 35/00 (2018.01); C07K 16/2818 (2013.01); C07K 16/30 (2013.01); A61K 2039/505 (2013.01); C07K 2317/54 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C07K 2317/75 (2013.01); C07K 2317/76 (2013.01)]

16 Claims

1. A particle, which can be a microparticle or nanoparticle, comprising two different targeting agents,

wherein each of the targeting agents is conjugated directly to the particle's surface,

wherein the two different targeting agents are not conjugated to each other,

wherein each of the targeting agents binds to a different protein receptor on a T cell surface, and

wherein the two different targeting agents are an antagonistic PD-1 antibody or active fragment thereof, and an agonistic OX40 antibody or active fragment thereof, respectively.