US 11,884,703 B2
Methods of processing a fluid including a recombinant therapeutic protein and use thereof
Tarl Vetter, Bridgewater, NJ (US); Michael Coolbaugh, Bridgewater, NJ (US); Veena Warikoo, Westford, MA (US); and Konstantin Konstantinov, Waban, MA (US)
Assigned to Genzyme Corporation, Cambridge, MA (US)
Filed by Genzyme Corporation, Cambridge, MA (US)
Filed on Feb. 19, 2021, as Appl. No. 17/180,272.
Application 17/180,272 is a division of application No. 15/677,888, filed on Aug. 15, 2017, granted, now 10,968,252.
Claims priority of provisional application 62/375,689, filed on Aug. 16, 2016.
Prior Publication US 2021/0171572 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/34 (2006.01); B01D 61/14 (2006.01); C07K 16/00 (2006.01); B01D 15/18 (2006.01)
CPC C07K 1/34 (2013.01) [B01D 15/1864 (2013.01); B01D 61/145 (2013.01); B01D 61/146 (2022.08); C07K 16/00 (2013.01); B01D 2315/06 (2013.01); C07K 2317/21 (2013.01); C07K 2317/31 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of processing a fluid comprising a recombinant therapeutic protein, the method comprising:
(a) providing a circuit system comprising (i) a tangential flow virus filtration (TFVF) unit having first and second inlets, and (ii) a conduit in fluid communication between the first and second inlets of the TFVF unit, comprising at least one port for flowing fluid into or out of, or both, of the circuit system,
wherein the circuit system is configured such that fluid can be flowed through the conduit and the TFVF unit, and filtrate comprising the recombinant therapeutic protein can be collected from the TFVF unit;
(b) flowing a fluid including a recombinant therapeutic protein into the circuit system through one of the at least one port, recirculating retentate in the circuit system, and collecting filtrate comprising the recombinant therapeutic protein from the TFVF unit for a period of time;
wherein rate of recirculating the retentate in the circuit system and rate of flowing the fluid into the circuit system are independently controlled.