US 11,884,702 B2
Systems and methods for process scale isolation of immunoglobulin G
Eugene Zurlo, Charleston, SC (US); Dennis Curtin, Charleston, SC (US); Klaus Peter Radtke, Apex, NC (US); Ryan Dorfman, Essex, VT (US); and Matthew Whelihan, Colchester, VT (US)
Assigned to Plasma Technologies, LLC, Charleston, SC (US)
Filed by Plasma Technologies, LLC, Charleston, SC (US)
Filed on Dec. 22, 2021, as Appl. No. 17/560,163.
Claims priority of provisional application 63/272,605, filed on Oct. 27, 2021.
Claims priority of provisional application 63/208,778, filed on Jun. 9, 2021.
Claims priority of provisional application 63/131,097, filed on Dec. 28, 2020.
Prior Publication US 2022/0204556 A1, Jun. 30, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/30 (2006.01); C07K 1/22 (2006.01); C07K 1/36 (2006.01); C07K 1/18 (2006.01); C07K 16/06 (2006.01)
CPC C07K 1/303 (2013.01) [C07K 1/22 (2013.01); C07K 1/36 (2013.01); C07K 16/065 (2013.01)] 18 Claims
 
1. A method for isolating a target protein from a solution comprising the target protein and a plurality of contaminants, comprising:
adding a salt to the solution to generate a supernatant and a precipitate;
dissolving the precipitate in an aqueous solution to generate a dissolved precipitate comprising the target protein and a first portion of the plurality of contaminants;
applying the dissolved precipitate to a first ion exchange media having a first charge with a first polarity under first buffer conditions selected to not bind the target protein to generate a first bound fraction comprising a second portion of the plurality of contaminants and a first flow-through, wherein the first flow-through comprises the target protein and a third portion of the plurality of contaminants;
preparing a second ion exchange media selected to bind both the target protein and a fourth portion of the plurality of contaminants under second buffer conditions, wherein the second ion exchange media comprises a second charge with a second polarity that is opposite that of the first polarity;
applying the first flow-through to the second ion exchange media under second buffer conditions to generate a second bound fraction comprising the fourth portion of the plurality of contaminants and a second flow-through comprising the target protein;
wherein preparing comprises selecting a limited capacity of the second exchange media such that greater than 70% of content of the target protein in the solution is recovered in the second flow-through.