US 11,884,701 B2
Chromatography membranes, devices containing them, and methods of use thereof
Damian Brellisford, Stoney Creek (CA); Donna Lisa Crossley, Hamilton (CA); Greg McIntosh, Toronto (CA); Robert Ruman, Cambridge (CA); John Rydall, Oakville (CA); and Christopher S. Shields, Windham, NH (US)
Assigned to Merck Millipore Ltd., Carrigtwohill (IE)
Filed by Merck Millipore Ltd., Carrigtwohill (IE)
Filed on Mar. 16, 2021, as Appl. No. 17/203,076.
Application 17/203,076 is a continuation of application No. 16/100,515, filed on Aug. 10, 2018, granted, now 10,981,949.
Application 16/100,515 is a continuation of application No. 15/427,447, filed on Feb. 8, 2017, granted, now 10,800,808.
Application 15/427,447 is a continuation of application No. 12/551,762, filed on Sep. 1, 2009, abandoned.
Claims priority of provisional application 61/102,797, filed on Oct. 3, 2008.
Claims priority of provisional application 61/093,600, filed on Sep. 2, 2008.
Prior Publication US 2021/0206801 A1, Jul. 8, 2021
Int. Cl. C07K 1/22 (2006.01); B01D 63/08 (2006.01); B01D 69/10 (2006.01); B01L 3/00 (2006.01); B01D 63/02 (2006.01); B01D 63/10 (2006.01); B01D 63/14 (2006.01); B01D 69/02 (2006.01); C07K 14/77 (2006.01); C07K 16/00 (2006.01); C12N 9/36 (2006.01); B01L 9/00 (2006.01)
CPC C07K 1/22 (2013.01) [B01D 63/024 (2013.01); B01D 63/034 (2022.08); B01D 63/082 (2013.01); B01D 63/087 (2013.01); B01D 63/10 (2013.01); B01D 63/14 (2013.01); B01D 69/02 (2013.01); B01D 69/106 (2022.08); B01L 3/502753 (2013.01); C07K 14/77 (2013.01); C07K 16/00 (2013.01); C12N 9/2462 (2013.01); C12Y 302/01017 (2013.01); B01D 2315/10 (2013.01); B01D 2325/36 (2013.01); B01L 9/527 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0409 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A fluid treatment device comprising:
a housing unit, wherein the housing unit comprises:
(a) an inlet, a retentate outlet, and a permeate outlet;
(b) a fluid flow path between the inlet and the retentate outlet; and
(c) a composite material within the housing unit, wherein the composite material comprises:
a support member comprising a plurality of pores extending through the support member; and
a non-self-supporting macroporous cross-linked gel comprising macropores having an average size of 10 nm to 3000 nm, said macroporous gel being located in the pores of the support member;
wherein:
the macroporous cross-linked gel is selected from the group consisting of N,N′-methylenebisacrylamide cross-linked copolymers and cross-linked polymers of isopropylacrylamide, dodecyl acrylate, ethyl acrylate, ethylene oxide, hydroxymethyl acrylate, n-butyl acrylate, N-(hydroxymethyl)acrylamide, N-methacryloylacrylamide, N-methyl-N-vinylacetamide, N,N-dimethylacrylamide, N-vinyl-pyrrolidone, octadecylacrylamide, octyl acrylate, propyl acrylate, stearyl acrylate, styrene, or vinyl alcohol, or a mixture thereof;
the cross-linked gel comprises functional groups, wherein said functional groups are Protein A;
said macropores of said macroporous cross-linked gel are smaller than said pores of said support member; and
the pores of the support member are substantially perpendicular to the fluid flow path from the inlet to the retentate outlet, and said fluid flow path from the inlet to the retentate outlet is substantially parallel to the surface of a filter medium comprising the composite material.