US 11,884,700 B2
Methods of inactivating viral contaminants
Filipa Abrantes, La Chaux-de-Fonds (CH); Sonia Letestu, La Chaux-de-Fonds (CH); Laure Cahuzac, La Chaux-de-Fonds (CH); and Lionel Duarte, La Chaux-de-Fonds (CH)
Assigned to ICHNOS SCIENCES SA, La Chaux-de-Fonds (CH)
Filed by ICHNOS SCIENCES SA, La Chaux-de-Fonds (CH)
Filed on Nov. 20, 2020, as Appl. No. 17/100,236.
Application 17/100,236 is a continuation of application No. 16/294,280, filed on Mar. 6, 2019, abandoned.
Application 16/294,280 is a continuation in part of application No. PCT/IB2018/056502, filed on Aug. 27, 2018.
Claims priority of application No. 17188012 (EP), filed on Aug. 25, 2017.
Prior Publication US 2021/0324000 A1, Oct. 21, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/22 (2006.01); C07K 1/18 (2006.01); C07K 16/06 (2006.01); A61K 39/395 (2006.01); C07K 16/00 (2006.01); C07K 1/34 (2006.01); C07K 1/36 (2006.01)
CPC C07K 1/22 (2013.01) [A61K 39/395 (2013.01); A61K 39/39591 (2013.01); C07K 1/18 (2013.01); C07K 1/34 (2013.01); C07K 1/36 (2013.01); C07K 16/00 (2013.01); C07K 16/065 (2013.01); C07K 2317/14 (2013.01); C07K 2317/31 (2013.01)] 14 Claims
 
1. A method for the removal of impurities from a clarified harvest antibody material, the method comprising: (a) culturing host cells which express an antibody, (b) harvesting the antibody material in the cell culture and (c) subjecting it to a clarification step, wherein said method further comprises (d) treating the resulting clarified harvest antibody material with high pH to reach a high target pH and (e) subjecting the high pH treated clarified harvest antibody material to a filtration step to remove impurities comprising host cell proteins, nucleic acids and/or cell debris wherein said high target pH is about 10.2 to about 11.0.