CPC C07K 1/1077 (2013.01) [B01D 15/1864 (2013.01); B01D 15/305 (2013.01); C07K 1/20 (2013.01); C07K 16/065 (2013.01)] | 11 Claims |
1. A method for preparing a target polypeptide from a mixture including the target polypeptide, the method comprising:
passing the mixture including the target polypeptide to a first column of a plurality of chromatographic columns in a hydrophobic interaction chromatography (HIC) apparatus, wherein each of the plurality of columns comprises an outlet connectable to another column of the plurality of columns;
passing an effluent including the target polypeptide from the first column to a second column of the plurality of columns;
passing one or more mobile phases to a third column of the plurality of columns; and
passing the target polypeptide through the outlets of each of the plurality of columns, wherein a sum of residence times for the mixture including the target polypeptide in the first column and second column is approximately the same as the sum of the residence times of the one or more mobile phases in the third column.
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