US 11,884,698 B2
Systems and methods for preparing a polypeptide from a mixture
Isabelle Livigni, New York, NY (US); Stefanie McDermott, Nyack, NY (US); James Reilly, Putnam Valley, NY (US); and John Mattila, Nyack, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Jul. 1, 2019, as Appl. No. 16/459,187.
Claims priority of provisional application 62/693,024, filed on Jul. 2, 2018.
Prior Publication US 2020/0002373 A1, Jan. 2, 2020
Int. Cl. B01D 15/18 (2006.01); B01D 15/30 (2006.01); C07K 1/20 (2006.01); C07K 1/107 (2006.01); C07K 16/06 (2006.01)
CPC C07K 1/1077 (2013.01) [B01D 15/1864 (2013.01); B01D 15/305 (2013.01); C07K 1/20 (2013.01); C07K 16/065 (2013.01)] 11 Claims
 
1. A method for preparing a target polypeptide from a mixture including the target polypeptide, the method comprising:
passing the mixture including the target polypeptide to a first column of a plurality of chromatographic columns in a hydrophobic interaction chromatography (HIC) apparatus, wherein each of the plurality of columns comprises an outlet connectable to another column of the plurality of columns;
passing an effluent including the target polypeptide from the first column to a second column of the plurality of columns;
passing one or more mobile phases to a third column of the plurality of columns; and
passing the target polypeptide through the outlets of each of the plurality of columns, wherein a sum of residence times for the mixture including the target polypeptide in the first column and second column is approximately the same as the sum of the residence times of the one or more mobile phases in the third column.