	US 11,884,644 B1
	Method for synthesizing (S)-nicotine and intermediate thereof
Kai Fu, Zibo (CN); Jiaquan Li, Zibo (CN); Peng Wang, Zibo (CN); Qinyuan Ma, Zibo (CN); Xicheng Zhang, Zibo (CN); Gengxiu Zheng, Zibo (CN); and Ruihua Hu, Zibo (CN)
Assigned to Shandong Jincheng Pharmaceutical Chemical Co., Ltd., Zibo (CN)
Filed by Shandong Jincheng Pharmaceutical Chemical Co., Ltd., Zibo (CN)
Filed on Sep. 1, 2023, as Appl. No. 18/460,072.
Claims priority of application No. 202310133879.6 (CN), filed on Feb. 20, 2023.
Int. Cl. C07D 401/04 (2006.01); C12P 17/12 (2006.01); C07D 213/38 (2006.01)

CPC C07D 401/04 (2013.01) [C07D 213/38 (2013.01); C12P 17/12 (2013.01); C07B 2200/07 (2013.01)]

20 Claims

1. A method for synthesizing a chiral intermediate (S)-4-amino-4-(3-pyridyl)-1-butanol for preparing (S)-nicotine, comprising:

performing a transamination reaction between 4-hydroxy-1-(3-pyridyl)-1-butanone and an amino donor to generate (S)-4-amino-4-(3-pyridyl)-1-butanol in catalysis of transaminase and coenzyme pyridoxal phosphate, the transaminase being one of transaminases with NCBI accession numbers being XP_007730450, 5FR9_A, WP_040602310 and XP_748821.