	US 11,884,566 B2
	Engineered yeast as a method for bioremediation
George Le-Le Sun, Culver City, CA (US); and Angela M. Belcher, Lexington, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Sep. 3, 2020, as Appl. No. 17/011,981.
Application 17/011,981 is a division of application No. 15/887,305, filed on Feb. 2, 2018, granted, now 10,766,798, issued on Sep. 8, 2020.
Claims priority of provisional application 62/453,609, filed on Feb. 2, 2017.
Prior Publication US 2021/0221719 A1, Jul. 22, 2021
Int. Cl. C02F 3/34 (2023.01); C02F 3/32 (2023.01); E21C 41/22 (2006.01); C07K 14/825 (2006.01); C12N 1/06 (2006.01); C12N 1/16 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); E21C 41/32 (2006.01); C22B 3/18 (2006.01); C02F 101/20 (2006.01); C02F 103/10 (2006.01)

CPC C02F 3/342 (2013.01) [C02F 3/322 (2013.01); C02F 3/347 (2013.01); C07K 14/825 (2013.01); C12N 1/063 (2013.01); C12N 1/16 (2013.01); C12N 9/0008 (2013.01); C12N 9/1029 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); E21C 41/22 (2013.01); E21C 41/32 (2013.01); C02F 2101/20 (2013.01); C02F 2101/203 (2013.01); C02F 2103/10 (2013.01); C07K 2319/03 (2013.01); C12Y 102/01011 (2013.01); C12Y 203/01031 (2013.01); C12Y 205/01047 (2013.01); C12Y 404/01008 (2013.01); C22B 3/18 (2013.01); Y02P 10/20 (2015.11)]

21 Claims

1. A composition for remediating a metal to treat water comprising:

an engineered yeast cell having a functionalized surface expressing a genetically engineered mutant membrane metal transporter, wherein the mutant membrane metal transporter has specificity for a metal and increases uptake;

a vacuole transporter; and

a metal sequestration protein.