US 11,884,024 B2
Systems and methods for forming a nanopore in a lipid bilayer
Roger J. A. Chen, Saratoga, CA (US); and Randy Davis, Pleasanton, CA (US)
Assigned to Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on May 18, 2021, as Appl. No. 17/303,024.
Application 17/303,024 is a division of application No. 16/179,800, filed on Nov. 2, 2018, granted, now 11,027,502.
Application 16/179,800 is a continuation of application No. 14/335,616, filed on Jul. 18, 2014, granted, now 10,456,993, issued on Oct. 29, 2019.
Application 14/335,616 is a continuation of application No. 12/658,591, filed on Feb. 8, 2010, granted, now 9,605,307, issued on Mar. 28, 2017.
Prior Publication US 2021/0268749 A1, Sep. 2, 2021
Int. Cl. B29C 67/20 (2006.01); B82Y 5/00 (2011.01); C12Q 1/6869 (2018.01); G01N 33/487 (2006.01); B82Y 10/00 (2011.01); B82Y 40/00 (2011.01)
CPC B29C 67/20 (2013.01) [B82Y 5/00 (2013.01); C12Q 1/6869 (2013.01); G01N 33/48721 (2013.01); B82Y 10/00 (2013.01); B82Y 40/00 (2013.01); Y10S 977/797 (2013.01); Y10S 977/90 (2013.01); Y10S 977/942 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A system for identifying a base of a nucleic acid molecule, comprising:
a sensing circuit configured to be coupled to a nanopore;
an electrical circuit configured to apply an electrical stimulus across the nanopore; and
a processor coupled to the sensing circuit, wherein the processor is programmed to:
control the sensing circuit to record a first electrical measurement indicative of a plurality of bases of the nucleic acid molecule as a strand of the nucleic acid molecule moves through the nanopore as a result of a first applied electrical stimulus;
control the electrical circuit to apply a second electrical stimulus across the nanopore that is configured to translocate the nucleic acid molecule through the nanopore;
control the sensing circuit to record a second electrical measurement indicative of the plurality of bases of the nucleic acid molecule as the strand of the nucleic acid molecule moves through the nanopore as a result of the second applied electrical stimulus, wherein the second electrical measurement is a repeat electrical measurement; and
identify the plurality of bases based on the first electrical measurement wherein the repeat measurement is used to increase confidence in the identification.