	US 11,883,535 B2
	Liposomal particles, methods of making same and uses thereof
Chad A. Mirkin, Wilmette, IL (US); Sonbinh T. Nguyen, Evanston, IL (US); Resham Singh Banga, Chicago, IL (US); Natalia Chernyak, Evanston, IL (US); Sergei Gryaznov, San Mateo, CA (US); Aleksandar Radovic-Moreno, Evanston, IL (US); and Christopher Mader, Cambridge, MA (US)
Assigned to NORTHWESTERN UNIVERSITY, Evanston, IL (US); and EXICURE, INC., Skokie, IL (US)
Filed by NORTHWESTERN UNIVERSITY, Evanston, IL (US); and EXICURE, INC., Skokie, IL (US)
Filed on Sep. 3, 2020, as Appl. No. 17/011,658.
Application 17/011,658 is a continuation of application No. 16/242,704, filed on Jan. 8, 2019, granted, now 10,792,251.
Application 16/242,704 is a continuation of application No. 15/101,523, granted, now 10,182,988, previously published as PCT/US2014/068429, filed on Dec. 3, 2014.
Claims priority of provisional application 61/982,269, filed on Apr. 21, 2014.
Claims priority of provisional application 61/911,334, filed on Dec. 3, 2013.
Prior Publication US 2021/0052497 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/127 (2006.01); A61K 48/00 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01)

CPC A61K 9/1271 (2013.01) [A61K 9/127 (2013.01); A61K 9/1277 (2013.01); A61K 48/00 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 2310/11 (2013.01); C12N 2310/12 (2013.01); C12N 2310/14 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01)]

23 Claims

1. A liposomal particle having a substantially spherical geometry, comprising:

a lipid bilayer comprising a plurality of lipid groups; and single-stranded oligonucleotides that are inhibitory RNA or antisense DNA, wherein all the oligonucleotides are attached to the surface of the liposomal particle, wherein each oligonucleotide is an oligonucleotide-lipid conjugate containing one lipophilic tethered group anchored into the external side of the lipid bilayer, wherein one or more of the oligonucleotides hybridize to a gene comprising a polynucleotide encoding a gene product and are complementary to all or a portion of the polynucleotide, wherein the hybridizing between the oligonucleotide and the polynucleotide occurs over a length of the polynucleotide with a degree of complementarity sufficient to inhibit expression of the gene product, and wherein the diameter of the liposomal particle is less than or equal to 50 nanometers (nm).

19. A liposomal particle having a substantially spherical geometry, consisting of:

a lipid bilayer consisting of a plurality of lipid groups; and single-stranded oligonucleotides that are inhibitory RNA or antisense DNA, wherein all the oligonucleotides are attached to the surface of the liposomal particle, wherein each oligonucleotide is an oligonucleotide-lipid conjugate containing one lipophilic tethered group anchored into the external side of the lipid bilayer, wherein one or more of the oligonucleotides hybridize to a gene comprising a polynucleotide encoding a gene product and are complementary to all or a portion of the polynucleotide, wherein the hybridizing between the polynucleotide and the oligonucleotide occurs over a length of the polynucleotide with a degree of complementarity sufficient to inhibit expression of the gene product, and wherein the diameter of the liposomal particle is less than or equal to 50 nanometers (nm).