	US 11,883,485 B2
	Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 Env using multimerized Env cores
Leonidas Stamatatos, Seattle, WA (US); Andrew McGuire, Seattle, WA (US); Katherine Rachael Parks, Seattle, WA (US); and Matthew D. Gray, Seattle, WA (US)
Assigned to Fred Hutchinson Cancer Center, Seattle, WA (US)
Appl. No. 17/269,199
Filed by Fred Hutchinson Cancer Center, Seattle, WA (US)
PCT Filed Aug. 29, 2019, PCT No. PCT/US2019/048825 § 371(c)(1), (2) Date Feb. 17, 2021, PCT Pub. No. WO2020/047263, PCT Pub. Date Mar. 5, 2020.
Claims priority of provisional application 62/724,555, filed on Aug. 29, 2018.
Prior Publication US 2021/0308256 A1, Oct. 7, 2021
Int. Cl. A61K 39/21 (2006.01); A61K 39/02 (2006.01); A61K 39/00 (2006.01)

CPC A61K 39/02 (2013.01) [A61K 2039/545 (2013.01); C12N 2740/16034 (2013.01); C12N 2740/16134 (2013.01)]

13 Claims

1. A method of eliciting antibodies that bind full length glycosylated human immunodeficiency virus (HIV)-1 envelope protein (Env) comprising administering to a subject:

(i) a prime immunization comprising a multimerized 426c core HIV-1 Env; and

(ii) a boost immunization comprising a multimerized HxB2 core HIV-1 Env with a glycosylated N276 wherein no Env are administered to the subject between the (i) prime immunization and (ii) boost immunization

thereby eliciting antibodies that bind full length glycosylated HIV-1 Env

wherein residue numbering is based on the prototypic HIV-1 isolate HxB2.