CPC A61K 35/36 (2013.01) [A61L 27/362 (2013.01); A61L 27/3687 (2013.01)] | 18 Claims |
1. A surgical method of using a graft comprising: implanting the graft during surgery, for treatment of a human patient; wherein the graft is prepared by:
(a) providing a bovine dermis having a native dermal matrix;
(b) treating the bovine dermis with an aqueous alkaline sodium sulfide solution for at least 1 hour, wherein the aqueous alkaline sodium sulfide solution comprises 0.01% to 1.5% by weight of sodium sulfide and 0.001 M to 0.5 M sodium hydroxide, wherein a pH of the aqueous alkaline sodium sulfide solution is greater than 10;
(c) treating the bovine dermis with a saline solution at least once;
(d) treating the bovine dermis with an aqueous hydrogen peroxide solution at least once;
(e) dehydrating the bovine dermis via solvent dehydration with a solvent starting with a first concentration of the solvent and finishing with a second concentration of the solvent, higher than the first concentration of the solvent; and
(f) maintaining a histoarchitecture of the native dermal matrix of the bovine dermis, wherein the graft is non-crosslinked.
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