US 11,882,808 B2
Methods for improving resistance to soybean rust
Marisa Miller, Durham, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Filed by Pairwise Plants Services, Inc., Durham, NC (US)
Filed on Mar. 25, 2021, as Appl. No. 17/212,686.
Claims priority of provisional application 63/000,608, filed on Mar. 27, 2020.
Prior Publication US 2021/0301283 A1, Sep. 30, 2021
Int. Cl. C12N 15/10 (2006.01); A01H 6/54 (2018.01); C12N 9/22 (2006.01); C07K 14/41 (2006.01); C07K 14/415 (2006.01)
CPC A01H 6/542 (2018.05) [C07K 14/415 (2013.01); C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12Y 499/01 (2013.01); C12N 2310/20 (2017.05)] 2 Claims
 
1. A soybean plant or plant part thereof comprising at least one non-natural mutation in each of an endogenous STAYGREEN1 (SGR1) gene that encodes an SGR1 protein and an endogenous STAYGREEN2 (SGR2) gene that encodes an SGR2 protein, wherein the at least one non-natural mutation is in the untranslated region or the promoter region of the endogenous SGR gene, and the at least one non-natural mutation in the SGR1 gene results in the nucleic acid sequence of any one of SEQ ID NOs:116, 119, 121, 124, 128, 129, 132, 135, 138, 139, 141, 142 or 144 and the at least one non-natural mutation in the SGR2 gene results in the nucleic acid sequence of any one of SEQ ID NOs:115, 117, 118, 120, 122, 125, 126, 127, 130, 131, 134, 136, 137, 140, or 143; and wherein the soybean plant or part thereof comprising the at least one non-natural mutation in the endogenous SGR1 gene and in the endogenous SGR2 gene exhibits an increase in resistance to soybean rust, delayed senescence and/or chlorophyll retention as compared to a control soybean plant or part thereof not comprising the at least one non-natural mutation in the endogenous SGR1 gene and in the endogenous SGR2 gene.