CPC C12Q 1/6869 (2013.01) [C07K 14/35 (2013.01); G01N 27/447 (2013.01)] | 18 Claims |
1. A method of sequencing a target polynucleotide, the method comprising:
(a) applying a potential difference across a pore in contact with a Hel308 helicase and the target polynucleotide;
(b) measuring at least two electrical signals for at least one nucleotide of the target polynucleotide moving through the pore during a full translocation cycle of the Hel308 helicase; and
(c) sequencing the target polynucleotide using the at least two electrical signals measured in (b).
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