US 11,879,155 B2
Compositions and methods for polynucleotide sequencing
Eric Stava, San Diego, CA (US); Jens H. Gundlach, Seattle, WA (US); Jeffrey G. Mandell, La Jolla, CA (US); Kevin L. Gunderson, Encinitas, CA (US); Ian M. Derrington, Seattle, WA (US); and Hosein Mohimani, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on May 25, 2021, as Appl. No. 17/329,482.
Application 17/329,482 is a continuation of application No. 16/439,022, filed on Jun. 12, 2019, granted, now 11,041,196.
Application 16/439,022 is a continuation of application No. 15/606,354, filed on May 26, 2017, granted, now 10,364,462, issued on Jul. 30, 2019.
Application 15/606,354 is a continuation of application No. 14/554,741, filed on Nov. 26, 2014, granted, now 9,689,033, issued on Jun. 27, 2017.
Claims priority of provisional application 61/909,316, filed on Nov. 26, 2013.
Prior Publication US 2021/0355534 A1, Nov. 18, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); G01N 27/447 (2006.01); C07K 14/35 (2006.01)
CPC C12Q 1/6869 (2013.01) [C07K 14/35 (2013.01); G01N 27/447 (2013.01)] 18 Claims
 
1. A method of sequencing a target polynucleotide, the method comprising:
(a) applying a potential difference across a pore in contact with a Hel308 helicase and the target polynucleotide;
(b) measuring at least two electrical signals for at least one nucleotide of the target polynucleotide moving through the pore during a full translocation cycle of the Hel308 helicase; and
(c) sequencing the target polynucleotide using the at least two electrical signals measured in (b).