CPC C12N 7/02 (2013.01) [C07K 1/36 (2013.01)] | 25 Claims |
1. A method of purifying a population of recombinant lentivirus particles, said method comprising:
obtaining a sample comprising the recombinant lentivirus particles and a contaminant;
subjecting the sample to a steric exclusion chromatography (SXC) column comprising a hydrophilic medium in the presence of polyethylene glycol (PEG) rendering the recombinant lentivirus particles bound to the hydrophilic medium;
eluting the SXC column with an elution buffer to obtain a preparation of the recombinant lentivirus particles; and
subjecting the preparation of the recombinant lentivirus particles to a multimodal chromatography column comprising a multimodal chromatographic medium which retains the contaminant;
collecting a flow-through liquid from the multimodal chromatography column, wherein the flow-through liquid comprises the recombinant lentivirus particles,
wherein the method does not comprise a step which retains the recombinant lentivirus particles to an ion exchange chromatography column.
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