US 11,879,139 B1
Scalable methods for purification of recombinant viruses
Dichao Hu, Jiangsu (CN); Chuanzhen Pi, Jiangsu (CN); Kai Weng, Jiangsu (CN); Jinbo Guo, Jiangsu (CN); Hailong Liu, Jiangsu (CN); Yangzhou Wang, Jiangsu (CN); Weimeng Zhang, Jiangsu (CN); and Jun Hua, Jiangsu (CN)
Assigned to PORTON ADVANCED SOLUTIONS LTD., Suzhou (CN)
Filed by PORTON ADVANCED SOLUTIONS LTD., Suzhou (CN)
Filed on Oct. 25, 2022, as Appl. No. 18/049,627.
Claims priority of application No. PCT/CN2022/119927 (WO), filed on Sep. 20, 2022.
Int. Cl. C12N 7/02 (2006.01); C07K 1/36 (2006.01)
CPC C12N 7/02 (2013.01) [C07K 1/36 (2013.01)] 25 Claims
 
1. A method of purifying a population of recombinant lentivirus particles, said method comprising:
obtaining a sample comprising the recombinant lentivirus particles and a contaminant;
subjecting the sample to a steric exclusion chromatography (SXC) column comprising a hydrophilic medium in the presence of polyethylene glycol (PEG) rendering the recombinant lentivirus particles bound to the hydrophilic medium;
eluting the SXC column with an elution buffer to obtain a preparation of the recombinant lentivirus particles; and
subjecting the preparation of the recombinant lentivirus particles to a multimodal chromatography column comprising a multimodal chromatographic medium which retains the contaminant;
collecting a flow-through liquid from the multimodal chromatography column, wherein the flow-through liquid comprises the recombinant lentivirus particles,
wherein the method does not comprise a step which retains the recombinant lentivirus particles to an ion exchange chromatography column.