	US 11,879,132 B2
	Plants having increased tolerance to herbicides
Raphael Aponte, Limburgerhof (DE); Stefan Tresch, Ludwigshafen (DE); Dario Massa, Limburgerhof (DE); Matthias Witschel, Ludwigshafen (DE); Tobias Seiser, Limburgerhof (DE); and Jill Marie Paulik, Research Triangle Park, NC (US)
Assigned to BASF AGRO B.V., Arnhem (NL)
Appl. No. 16/468,036
Filed by BASF AGRO B.V., Arnhem (NL)
PCT Filed Dec. 18, 2017, PCT No. PCT/EP2017/083244 § 371(c)(1), (2) Date Jun. 10, 2019, PCT Pub. No. WO2018/114759, PCT Pub. Date Jun. 28, 2018.
Claims priority of application No. 16205383 (EP), filed on Dec. 20, 2016.
Prior Publication US 2020/0270625 A1, Aug. 27, 2020
Int. Cl. C12N 15/82 (2006.01); A01H 6/54 (2018.01); A01H 6/46 (2018.01); A01H 6/20 (2018.01); C07K 14/195 (2006.01)

CPC C12N 15/8274 (2013.01) [C07K 14/195 (2013.01); A01H 6/20 (2018.05); A01H 6/4684 (2018.05); A01H 6/542 (2018.05)]

28 Claims

1. A plant or plant part comprising a polynucleotide encoding a mutated PPO polypeptide having at least 80% identity with the amino acid sequence of the PPO polypeptide sequence of SEQ ID NO: 1, wherein the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides, wherein said mutated PPO polypeptide comprises one or more of the following motifs:

a) Motif 2:

[A/S/C]PS[D/N][X][X]L (SEQ ID NO: 132)

wherein the serine at position 3 within said motif is substituted by any other amino acid

b) Motif 3:

[R/Q][E/D]KQQ[L/Y]P (SEQ ID NO: 133)

wherein the glutamine at position 4 within said motif is substituted by any other amino acid

c) Motif 4:

L[I/V]PSKE (SEQ ID NO: 134)

wherein the serine at position 4 within said motif is substituted by any other amino acid.