US 11,879,129 B2
Modulation of transgene expression in plants
Andrew J. Bowling, Zionsville, IN (US); Marcelo Ariel German, Lake Oswego, OR (US); Todd P. Glancy, Fairmount, IN (US); Sandeep Kumar, Johnston, IA (US); Heather Pence, Indianapolis, IN (US); Andrew E. Robinson, Calvert City, KY (US); Shreedharan Sriram, Waukee, IA (US); Po-Hao Wang, Cary, NC (US); and Carla N. Yerkes, Crawfordsville, IN (US)
Filed by CORTEVA AGRISCIENCE LLC, Indianapolis, IN (US)
Filed on Sep. 12, 2022, as Appl. No. 17/931,436.
Application 16/856,536 is a division of application No. 15/705,312, filed on Sep. 15, 2017, abandoned.
Application 17/931,436 is a continuation of application No. 16/856,536, filed on Apr. 23, 2020, granted, now 11,473,095.
Claims priority of provisional application 62/406,560, filed on Oct. 11, 2016.
Prior Publication US 2023/0136893 A1, May 4, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8218 (2013.01) [C12N 15/8223 (2013.01); C12N 15/8234 (2013.01); C12N 15/8274 (2013.01)] 14 Claims
1. A method for modulating the expression of transgene expression in tissue of plants, the method comprising the steps of:
a. Providing at least one gene expression cassette comprising a sRNA target site of SEQ ID NO:4 operably linked to a transgene into a plant; and,
b. Expressing the transgene, wherein the modulation of the transgene expression occurs in seed-coat specific plant tissue and the transgene is expressed in the other plant tissues.