	US 11,878,312 B2
	Centrifuge system for separating cells in suspension
Stephen B. Kessler, Princeton, MA (US); and T. David Marro, Magnolia, MA (US)
Assigned to PNEUMATIC SCALE CORPORATION, Cuyahoga Falls, OH (US)
Filed by Pneumatic Scale Corporation, Cuyahoga Falls, OH (US)
Filed on Jan. 28, 2022, as Appl. No. 17/587,783.
Application 17/587,783 is a continuation of application No. 17/323,154, filed on May 18, 2021.
Application 17/323,154 is a continuation of application No. 16/433,180, filed on Jun. 6, 2019, granted, now 11,065,629, issued on Jul. 20, 2021.
Application 16/433,180 is a continuation in part of application No. 16/003,659, filed on Jun. 8, 2018, granted, now 11,033,911, issued on Jun. 15, 2021.
Application 16/003,659 is a continuation in part of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 15/886,382 is a continuation of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 15/886,382 is a continuation in part of application No. 14/698,995, filed on Apr. 29, 2015, abandoned.
Application 14/698,995 is a continuation of application No. 13/684,051, filed on Nov. 21, 2012, granted, now 9,222,067, issued on Dec. 29, 2015.
Claims priority of provisional application 62/682,376, filed on Jun. 8, 2018.
Claims priority of provisional application 61/562,438, filed on Nov. 21, 2011.
Prior Publication US 2022/0152632 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B04B 11/04 (2006.01); B04B 11/02 (2006.01); B04B 11/08 (2006.01); B04B 5/04 (2006.01)

CPC B04B 11/04 (2013.01) [B04B 11/02 (2013.01); B04B 11/082 (2013.01); B04B 2005/0464 (2013.01)]

43 Claims

1. Apparatus comprising:

a single use structure configured for use in a centrifuge system including a multi-use rotatable centrifuge bowl, wherein the single use structure is configured to be positioned in the bowl and to separate within an interior area of the single use structure, cells in a cell culture batch into concentrate and centrate,

the single use structure in an operative position, including

an upper disc shape portion, wherein the upper disc shape portion extends in centered relation about a vertically extending axis,

a lower portion,

an annular axially centered radially extending core, wherein the core extends axially intermediate of the upper portion and the lower portion,

a separation chamber in radially surrounding relation of the core,

an outer wall, wherein the outer wall

extends in fluid tight operative relation with the upper disc shape portion and bounds the separation chamber,

extends in surrounding relation of the core, and has an internal shape with a smaller inside radius adjacent the lower portion,

wherein the upper disc shape portion and the outer wall are rotatable within the bowl about the axis,

an axially centered vertically extending central tube, wherein the central tube is configured to pass cells therethrough,

a vertically extending centrate discharge tube,

a centrate centripetal pump, wherein the centrate centripetal pump is

disposed coaxially with the core,

disposed coaxially about the central tube,

and in fluid communication with the centrate discharge tube,

wherein the centrate centripetal pump is positioned in the interior area in a centrate centripetal pump chamber, wherein the centrate centripetal pump chamber is in fluid communication with the separation chamber,

wherein during rotation of the bowl, the upper disc shape portion and the outer wall rotate relative to each of the central tube, the centrate discharge tube and the centrate centripetal pump.