US 11,878,311 B2
Centrifuge system for separating cells in suspension
Stephen B. Kessler, Princeton, MA (US); and T. David Marro, Magnolia, MA (US)
Assigned to PNEUMATIC SCALE CORPORATION, Cuyahoga Falls, OH (US)
Filed by Pneumatic Scale Corporation, Cuyahoga Falls, OH (US)
Filed on Jan. 28, 2022, as Appl. No. 17/587,691.
Application 17/587,691 is a continuation of application No. 17/323,154, filed on May 18, 2021.
Application 17/323,154 is a continuation of application No. 16/433,180, filed on Jun. 6, 2019, granted, now 11,065,629, issued on Jul. 20, 2021.
Application 16/433,180 is a continuation in part of application No. 16/003,659, filed on Jun. 8, 2018, granted, now 11,033,911, issued on Jun. 15, 2021.
Application 16/003,659 is a continuation in part of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 16/003,659 is a continuation of application No. 15/886,382, filed on Feb. 1, 2018, granted, now 10,384,216, issued on Aug. 20, 2019.
Application 15/886,382 is a continuation in part of application No. 14/698,995, filed on Apr. 29, 2015, abandoned.
Application 14/698,995 is a continuation of application No. 13/684,051, filed on Nov. 21, 2012, granted, now 9,222,067, issued on Dec. 29, 2015.
Claims priority of provisional application 62/682,376, filed on Jun. 8, 2018.
Claims priority of provisional application 61/562,438, filed on Nov. 21, 2011.
Prior Publication US 2022/0143627 A1, May 12, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B04B 11/04 (2006.01); B04B 11/02 (2006.01); B04B 11/08 (2006.01); B04B 5/04 (2006.01)
CPC B04B 11/04 (2013.01) [B04B 11/02 (2013.01); B04B 11/082 (2013.01); B04B 2005/0464 (2013.01)] 24 Claims
OG exemplary drawing
 
1. Apparatus comprising:
a single use structure configured for use in a centrifuge system including a multi-use rotatable centrifuge bowl, wherein the structure is configured to be removably positioned in the bowl and to separate within an interior area of the structure, cells in a cell culture batch into concentrate and centrate,
the structure in an operative position, including
an upper disc shape portion,
a lower portion,
a cylindrical core intermediate of the upper portion and the lower portion,
a separation chamber in radially surrounding relation of the core,
an outer wall, wherein the outer wall
extends in fluid tight operative relation with the upper disc shape portion and bounds the separation chamber,
extends in surrounding relation of the core, and
has an internal truncated cone shape with a smaller inside radius adjacent the lower portion,
a vertically extending feed tube,
a vertically extending centrate discharge tube,
wherein the upper disc shape portion and the outer wall are rotatable within the bowl about a vertical axis,
a centrate centripetal pump, wherein the centrate centripetal pump is
axially aligned with the core,
disposed coaxially about the feed tube,
in fluid communication with the centrate discharge tube, and
positioned in a centrate pump chamber, wherein the centrate pump chamber is in fluid communication with the separation chamber,
wherein during rotation of the bowl, the upper disc shape portion and the outer wall rotate relative to each of the feed tube, the centrate discharge tube and the centrate centripetal pump.