US 11,878,082 B2
Biomass-based encapsulating material for protection of probiotic activity and an encapsulating method
Junling Guo, Chengdu (CN); Yaoyao Zhang, Chengdu (CN); Jiezhou Pan, Chengdu (CN); and Guidong Gong, Chengdu (CN)
Assigned to CHENGDU BANGJIALEJUN BIOTECHNOLOGY CO., LTD, Chengdu (CN)
Filed by Chengdu bangjialejun Biotechnology Co., Ltd, Chengdu (CN)
Filed on Sep. 21, 2021, as Appl. No. 17/481,263.
Claims priority of application No. 202110770726.3 (CN), filed on Jul. 8, 2021.
Prior Publication US 2022/0000793 A1, Jan. 6, 2022
Int. Cl. A61K 9/51 (2006.01); A61K 35/741 (2015.01); A61K 9/50 (2006.01)
CPC A61K 9/5176 (2013.01) [A61K 9/5073 (2013.01); A61K 9/5115 (2013.01); A61K 9/5169 (2013.01); A61K 35/741 (2013.01)] 8 Claims
OG exemplary drawing
 
1. An encapsulated probiotic comprising:
a probiotics solution, wherein the probiotics solution has a concentration between 1*106 CFU/mL and 1*109 CFU/mL;
a biomass-based encapsulating material for protection of probiotic activity of the probiotics solution, the biomass-based encapsulating material comprising a nano-film, the nano-film comprising:
a first layer formed from covalent cross-linking or metal chelating action in situ between metal ions of a metal salt aqueous solution and biological macromolecules of a macromolecular aqueous solution, wherein the metal ions are selected from the group consisting of cations of Al, Fe, Zn, Mn, Ni, Co and V and combinations thereof, wherein the biological macromolecules are selected from the group consisting of polyphenol, dopamine, dopamine derivatives, polysaccharide biomass, and combinations thereof, wherein the metal salt aqueous solution has a concentration between 1 mg/mL and 10 mg/mL, wherein a volume ratio of the metal salt aqueous solution to the probiotics solution is 1:50, wherein the macromolecular aqueous solution has a concentration between 10 mg/mL and 100 mg/mL, wherein a volume ratio of the macromolecular aqueous solution to the probiotics solution is 1:50; and
a second layer formed by interactions between bio-enzymes of a bio-enzyme solution and the biological macromolecules of the macromolecular aqueous solution, wherein the bio-enzymes are selected from the group consisting of β-galactosidase, amylopsin, α-glucosaccharase, β-glucosaccharase, pancreatic lipase, trypsin, cellulose, and combinations thereof, wherein the bio-enzyme solution has a concentration between 1 mg/mL and 20 mg/mL, wherein a volume ratio of the bio-enzyme aqueous solution to the probiotics solution is 1:50.