	US 11,878,066 B2
	Composition and method for detection of demineralisation
Emma Perfect, Edinburgh (GB); and Chris Longbottom, Newport-on-Tay (GB)
Assigned to Calcivis Limited, Edinburgh (GB)
Filed by Calcivis Limited, Edinburgh (GB)
Filed on Dec. 28, 2017, as Appl. No. 15/857,470.
Application 15/857,470 is a division of application No. 12/520,128, granted, now 10,441,667, previously published as PCT/GB2007/004944, filed on Dec. 21, 2007.
Claims priority of application No. 0625678 (GB), filed on Dec. 21, 2006.
Prior Publication US 2018/0243449 A1, Aug. 30, 2018
Int. Cl. A61K 49/00 (2006.01)

CPC A61K 49/006 (2013.01)

22 Claims

1. A method for the intra-oral detection of active dental caries and/or active tooth demineralisation due to erosion, comprising the steps of:

exposing a tooth to a pharmaceutical composition comprising proteins or protein complexes which produce a bioluminescent optical signal in the presence of free ions, wherein the protein or protein complex comprises aequorin, obelin, clytin, mitrocomin, halistaurin, phialidin, mnemiopsin, symplectin, gr-bolinopsin, casein, calsequestrin, calexcitin, calcium binding cysteine protease, berovin, or mixtures thereof at a concentration that gives a detectably high signal to noise ratio, and

detecting the resulting bioluminescent optical signal intra-orally.