| CPC G02B 21/367 (2013.01) [G02B 21/14 (2013.01); G06T 7/0012 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01)] | 20 Claims |
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1. A method including:
receiving a plurality of images captured using structured illumination microscopy (SIM) in an optical system, each image of the plurality of images having a first field of view;
defining a window, the window defining a second field of view representing a portion of the first field of view such that the second field of view is smaller than the first field of view;
moving the window in relation to each image of a plurality of images;
capturing a plurality of sub-tiles from each image of the plurality of images while moving the window in relation to each image of the plurality of images,
each sub-tile of the plurality of plurality of sub-tiles representing a portion of the corresponding image of the plurality of images, the portion represented by each sub-tile of the plurality of sub-tiles being defined by the second field of view at a position corresponding to a moment at which the sub-tile of the plurality of sub-tiles is captured;
estimating parameters associated with each sub-tile of the plurality of sub-tiles, the parameters comprising an angle, a spacing, and a phase offset; and
storing the estimated parameters in a predetermined format.
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