US 12,203,938 B2
Levetiracetam immunoassays
Johnny Jose Valdez, Fremont, CA (US); Byung Sook Moon, Palo Alto, CA (US); Ki Chung, Fremont, CA (US); and Alejandro A. Orozco, Gilroy, CA (US)
Assigned to ARK Diagnostics, Inc., Fremont, CA (US)
Filed by ARK Diagnostics, Inc., Fremont, CA (US)
Filed on Dec. 10, 2021, as Appl. No. 17/548,239.
Application 17/548,239 is a division of application No. 16/720,886, filed on Dec. 19, 2019, granted, now 11,231,424.
Application 13/427,764 is a division of application No. 12/604,249, filed on Oct. 22, 2009, granted, now 8,168,756.
Application 16/720,886 is a continuation of application No. 14/459,221, filed on Aug. 13, 2014, abandoned.
Application 14/459,221 is a continuation of application No. 13/427,764, filed on Mar. 22, 2012, granted, now 8,841,136.
Claims priority of provisional application 61/108,369, filed on Oct. 24, 2008.
Prior Publication US 2022/0357331 A1, Nov. 10, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); C07D 207/26 (2006.01); C07K 16/44 (2006.01); G01N 33/94 (2006.01)
CPC G01N 33/581 (2013.01) [C07D 207/26 (2013.01); C07K 16/44 (2013.01); G01N 33/9473 (2013.01); G01N 2458/00 (2013.01)] 8 Claims
 
1. A method for detecting levetiracetam, the method comprising:
combining in a reaction mixture (a) a sample suspected of containing levetiracetam, (b) at least one polyclonal anti-levetiracetam antibody and (c) a compound of Formula 1:

OG Complex Work Unit Chemistry
wherein:
R1 is selected from the group consisting of —(CH2)n—NH—C(O)—(CH2)n—C(O)—Z and —(CH2)n—NH—C(O)—(CH2)n—S—(CH2)n—C(O)—Z, wherein each n is an integer from 1 to 10; and
Z is an enzyme, and
acid salts thereof; and
measuring the activity of the enzyme, whereby the activity of the enzyme indicates the presence of levetiracetam in the sample.