US 12,203,926 B2
Oral health model for high throughput screen and characterization of oral hygiene products
Camille Zenobia, Hampton, NJ (US); Harsh Mahendra Trivedi, Hillsborough, NJ (US); Ying Yang, Monmouth Junction, NJ (US); Carlo Daep, Brooklyn, NY (US); and James Masters, Ringoes, NJ (US)
Assigned to Colgate-Palmolive Company, New York, NY (US)
Appl. No. 17/309,896
Filed by Colgate-Palmolive Company, New York, NY (US)
PCT Filed Dec. 17, 2019, PCT No. PCT/US2019/066791
§ 371(c)(1), (2) Date Jun. 28, 2021,
PCT Pub. No. WO2020/139617, PCT Pub. Date Jul. 2, 2020.
Claims priority of provisional application 62/785,097, filed on Dec. 26, 2018.
Prior Publication US 2022/0091101 A1, Mar. 24, 2022
Int. Cl. G01N 33/50 (2006.01); C12N 1/20 (2006.01); G09B 23/30 (2006.01); A61K 8/27 (2006.01); A61Q 11/00 (2006.01); C12R 1/04 (2006.01); C12R 1/46 (2006.01)
CPC G01N 33/5008 (2013.01) [C12N 1/20 (2013.01); G09B 23/306 (2013.01); A61K 8/27 (2013.01); A61Q 11/00 (2013.01); C12N 2513/00 (2013.01); C12N 2533/18 (2013.01); C12R 2001/04 (2021.05); C12R 2001/46 (2021.05)] 9 Claims
OG exemplary drawing
 
1. An oral biology model comprising:
a) biofilm that comprises oral bacteria, wherein the biofilm is produced by culturing oral bacteria on a solid substrate;
b) oral epithelial tissue selected from the group consisting of gingival epithelial tissue and buccal epithelial tissue;
c) a suspension of neutrophil-like cells in media that comprises neutrophil-like cells that are differentiated HL60 cells induced to a neutrophil-like phenotype by treatment with retinoic acid;
wherein the oral epithelial tissue is layered on the suspension of neutrophil-like cells in media and the biofilm is layered on the oral epithelial tissue, and the biofilm is not in contact with the suspension of and is isolated from the neutrophil-like cells.