US 12,203,126 B2
System and method for clarifying a cell culture harvest solution
Vasily Medvedev, Nivelles (BE); Tiago Albano, Grimbergen (BE); and José Castillo, Brussels (BE)
Assigned to EXOTHERA S.A., Nivelles (BE)
Appl. No. 17/054,578
Filed by EXOTHERA S.A., Nivelles (BE)
PCT Filed May 13, 2019, PCT No. PCT/EP2019/062227
§ 371(c)(1), (2) Date Nov. 11, 2020,
PCT Pub. No. WO2019/215344, PCT Pub. Date Nov. 14, 2019.
Claims priority of provisional application 62/827,009, filed on Mar. 30, 2019.
Claims priority of provisional application 62/670,220, filed on May 11, 2018.
Prior Publication US 2021/0189454 A1, Jun. 24, 2021
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12Q 1/24 (2006.01)
CPC C12Q 1/24 (2013.01) [C12M 29/04 (2013.01); C12M 29/14 (2013.01); C12M 47/02 (2013.01); C12M 47/10 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A system for clarifying a cell culture harvest solution, including target molecules and dynamic filter media, comprising:
a filtration vessel comprising an outer container which is rigid or semi-rigid, a flexible liner contained in the outer container, and an outlet;
the filtration vessel including at least one filter located within the flexible liner, each filter having a lateral side surface,
a pump or actuator adapted to create a pressure differential which generates a flow perpendicular to the lateral side surface of each filter and causes the dynamic filter media to accumulate into a cake on the lateral side surface of each filter, said cake and at least one filter adapted, during filtration operation, to permit a filtrate including the target molecules to pass therethrough and said cake, during filtration operation, adapted to prevent unwanted solid materials from passing therethrough; and
a backflush source including a backflush fluid and fluidly connected to the filtration vessel via the at least one filter, said backflush source, during backflush operation, adapted to supply backflush fluid back through the at least one filter and cake accumulated on the lateral side surface of each filter, for removing the cake formed on the at least one filter, wherein the cake removed from the at least one filter is discharged through the outlet in the filtration vessel.