	US 12,203,117 B2
	Method of producing sulfur-containing amino acid or derivative thereof
Sol Choi, Seoul (KR); Hee Ju Kim, Seoul (KR); Jin Ah Rho, Seoul (KR); Jin Nam Lee, Seoul (KR); Han Hyoung Lee, Seoul (KR); Sun Young Lee, Seoul (KR); Sang Jun Kim, Seoul (KR); and Jihyun Shim, Seoul (KR)
Assigned to CJ Cheiljedang Corporation, Seoul (KR)
Appl. No. 17/597,019
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Jun. 26, 2020, PCT No. PCT/KR2020/008412 § 371(c)(1), (2) Date Dec. 22, 2021, PCT Pub. No. WO2020/263041, PCT Pub. Date Dec. 30, 2020.
Claims priority of application No. 10-2019-0077998 (KR), filed on Jun. 28, 2019.
Prior Publication US 2022/0315964 A1, Oct. 6, 2022
Int. Cl. C12P 13/12 (2006.01); C07K 14/245 (2006.01); C07K 14/34 (2006.01); C12N 15/70 (2006.01); C12N 15/77 (2006.01)

CPC C12P 13/12 (2013.01) [C07K 14/245 (2013.01); C07K 14/34 (2013.01); C12N 15/70 (2013.01); C12N 15/77 (2013.01)]

12 Claims

1. A method of producing a sulfur-containing amino acid or a derivative of the sulfur-containing amino acid, the method comprising culturing a genetically modified microorganism in a culture medium including thiosulfate,

wherein the microorganism includes genetic modification to increase an expression level of a protein encoded by ssuABC gene compared to a non-modified microorganism,

wherein the protein encoded by ssuABC gene is a complex of SsuA, SsuB, and SsuC proteins, and wherein the sulfur-containing amino acid or the derivative of the sulfur-containing amino acid is selected from the group consisting of methionine, cysteine, cystine, lanthionine, homocysteine, homocystine, homolanthionine, and taurine, and

wherein the microorganism has an increase in an expression level of at least one protein selected from the group consisting of SsuA, SsuB, and SsuC proteins compared to a non-modified microorganism.