	US 12,203,111 B2
	*Materials and methods for creating strains of Saccharomyces cerevisiae* that exhibit an increased ability to ferment oligosaccharides into ethanol**
Mark Goebl, Indianapolis, IN (US); Joshua Heyen, Brownsburg, IN (US); Nadaraj Palaniappan, Carmel, IN (US); Ross Cocklin, Keene, NH (US); Kathryn Houin, Lebanon, IN (US); and Matthew Kelker, Zionsville, IN (US)
Assigned to Xylogenics, Inc., Indianapolis, IN (US)
Appl. No. 17/261,454
Filed by Xylogenics, Inc., Indianapolis, IN (US); Mark Goebl, Indianapolis, IN (US); Joshua Heyen, Brownsburg, IN (US); Nadaraj Palaniappan, Carmel, IN (US); Ross Cocklin, Keene, NH (US); Kathryn Houin, Lebanon, IN (US); and Matthew Kelker, Zionsville, IN (US)
PCT Filed Jul. 19, 2019, PCT No. PCT/US2019/042605 § 371(c)(1), (2) Date Jan. 19, 2021, PCT Pub. No. WO2020/018905, PCT Pub. Date Jan. 23, 2020.
Claims priority of provisional application 62/700,679, filed on Jul. 19, 2018.
Prior Publication US 2021/0309982 A1, Oct. 7, 2021
Int. Cl. C12N 9/24 (2006.01); C12N 15/52 (2006.01)

CPC C12N 9/2402 (2013.01) [C12N 15/52 (2013.01); C12Y 302/0102 (2013.01)]

8 Claims

1. A recombinant yeast strain comprising a strain of S. cerevisiae and an exogenous MAL1 gene cluster comprising a MAL11 gene, a MAL12 gene, and/or a MAL13 gene, wherein the strain of S. cerevisiae expresses the exogenous MAL1 gene cluster, wherein the exogenous MAL1 gene cluster comprises a sequence having at least 80, 85, 90, 95, or 98 percent homology to SEQ ID NO: 2; and wherein the recombinant yeast strain further comprises an exogenous MAL2-8c gene cluster, wherein the exogenous MAL2-8c gene cluster comprises a sequence having at least 80, 85, 90, 95, or 98 percent homology SEQ ID NO: 3.