	US 12,203,108 B2
	Psicose-6-phosphate phosphatase, composition for producing psicose including said enzyme, method for producing psicose using said enzyme
Byung-Sam Son, Ansan-Si (KR); Hyun Kug Cho, Suwon-Si (KR); Sung Jae Yang, Suwon-Si (KR); Seong Bo Kim, Seongnam-Si (KR); Seung Hwan Kim, Seoul (KR); and Hyun June Park, Suwon-Si (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 16/635,474
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Jul. 25, 2018, PCT No. PCT/KR2018/008396 § 371(c)(1), (2) Date Jan. 30, 2020, PCT Pub. No. WO2019/027173, PCT Pub. Date Feb. 7, 2019.
Claims priority of application No. 10-2017-0097334 (KR), filed on Jul. 31, 2017.
Prior Publication US 2021/0355461 A1, Nov. 18, 2021
Int. Cl. C12N 9/16 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/26 (2006.01); C12N 9/34 (2006.01); C12N 9/44 (2006.01); C12N 9/90 (2006.01); C12P 19/02 (2006.01)

CPC C12N 9/16 (2013.01) [C12N 9/1051 (2013.01); C12N 9/1205 (2013.01); C12N 9/2414 (2013.01); C12N 9/2428 (2013.01); C12N 9/2457 (2013.01); C12N 9/246 (2013.01); C12N 9/90 (2013.01); C12P 19/02 (2013.01); C12Y 204/01001 (2013.01); C12Y 204/01007 (2013.01); C12Y 207/01002 (2013.01); C12Y 301/03025 (2013.01); C12Y 501/03 (2013.01); C12Y 503/01009 (2013.01); C12Y 504/02002 (2013.01)]

15 Claims

1. A method for producing D-psicose comprising contacting a psicose-6-phosphate phosphatase, a microorganism expressing the psicose-6-phosphate phosphatase or a culture of the microorganism expressing the psicose-6-phosphate phosphatase with D-psicose-6-phosphate to convert the D-psicose-6-phosphate to D-psicose;

wherein the psicose-6-phosphate phosphatase comprises:

i) a motif A comprising at least one sequence selected from the group consisting of SEQ ID NOS: 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, and 91;

ii) a motif B comprising a sequence having at least 90% sequence identity to at least one sequence selected from the group consisting of SEQ ID NOS: 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102,103, 104, 105, 106, 107, and 108; and

iii) A remainder sequence comprising remainder having at least 85% sequence identity to the amino acids sequence other than motif A and/or motif B in any sequence selected from the group consisting of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20.