US 12,203,087 B2
Enzymes and methods for producing omega-3 fatty acids
James Robertson Petrie, Bywong (AU); Anne Maree Mackenzie, Hawker (AU); Qing Liu, Giralang (AU); Pushkar Shrestha, Lawson (AU); Peter David Nichols, West Hobart (AU); Susan Irene Ellis Blackburn, Battery Point (AU); Maged Peter Mansour, Gladstone Park (AU); Stanley Suresh Robert, Oyster Cove (AU); Dion Matthew Frederick Frampton, Oyster Cove (AU); Xue-Rong Zhou, Bonnet Hill Harrison (AU); Surinder Pal Singh, Downer (AU); and Craig Christopher Wood, Dickson (AU)
Assigned to COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, Acton (AU); and GRAINS RESEARCH AND DEVELOPMENT CORPORATION, Barton (AU)
Filed by Commonwealth Scientific and Industrial Research Organisation, Acton (AU); and GRAINS RESEARCH AND DEVELOPMENT CORPORATION, Barton (AU)
Filed on Jan. 10, 2024, as Appl. No. 18/409,596.
Application 18/409,596 is a continuation of application No. 16/860,936, filed on Apr. 28, 2020, granted, now 11,976,287.
Application 16/860,936 is a continuation of application No. 15/642,223, filed on Jul. 5, 2017, granted, now 10,648,046, issued on May 12, 2020.
Application 15/642,223 is a continuation of application No. 14/332,202, filed on Jul. 15, 2014, granted, now 9,938,486, issued on Apr. 10, 2018.
Application 14/332,202 is a continuation of application No. 13/129,940, granted, now 8,809,559, issued on Aug. 19, 2014, previously published as PCT/AU2009/001488, filed on Nov. 17, 2009.
Claims priority of provisional application 61/270,710, filed on Jul. 9, 2009.
Prior Publication US 2024/0150780 A1, May 9, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C12P 7/6427 (2022.01); C12P 7/6432 (2022.01); C12P 7/6434 (2022.01); C12P 7/6472 (2022.01)
CPC C12N 15/8247 (2013.01) [C12P 7/6427 (2013.01); C12P 7/6432 (2022.01); C12P 7/6434 (2022.01); C12P 7/6472 (2013.01); C12Y 602/01003 (2013.01)] 14 Claims
 
1. A process for producing oil comprising eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA), the process comprising the steps of (i) obtaining seed of Brassicaceae plant, the seed comprising a total fatty acid content and exogenous polynucleotides encoding a microalgal Δ6-desaturase which has greater Δ6-desaturase activity on α-linolenic acid (ALA) joined to CoA (ALA-CoA) as fatty acid substrate than on ALA joined to the sn-2 position of phosphatidylcholine (PC) as the fatty acid substrate, a Δ6-elongase, and a microalgal Δ5-desaturase, wherein each polynucleotide is operably linked to one or more promoters that direct expression of said polynucleotides during the development of the seed, wherein ALA, stearidonic acid (SDA), eicosatetraenoic acid (ETA), EPA and DPA are each present at a level in the total fatty acid content of the seed, each level being expressed as a percentage of the total fatty acid content, such that the sum of the percentages for EPA and DPA divided by the sum of the percentages for ALA, SDA, ETA, EPA and DPA, expressed as a percentage, is at least 17.3%, and
(ii) extracting oil from the seed, thereby producing the oil comprising EPA and DPA.