US 12,203,059 B2
Microwell design and fabrication for generation of cell culture aggregates
Ye Fang, Painted Post, NY (US); Ann MeeJin Ferrie, Salem, NH (US); Vasiliy Nikolaevich Goral, Painted Post, NY (US); Allison Jean Tanner, Portsmouth, NH (US); and Qi Wu, Painted Post, NY (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by CORNING INCORPORATED, Corning, NY (US)
Filed on Nov. 22, 2021, as Appl. No. 17/532,196.
Application 17/532,196 is a continuation of application No. 15/499,370, filed on Apr. 27, 2017, abandoned.
Application 15/499,370 is a continuation of application No. PCT/US2015/058123, filed on Oct. 29, 2015.
Claims priority of provisional application 62/072,019, filed on Oct. 29, 2014.
Prior Publication US 2022/0081661 A1, Mar. 17, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12M 1/34 (2006.01); C12N 5/00 (2006.01)
CPC C12M 23/12 (2013.01) [C12M 41/36 (2013.01); C12N 5/0062 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A cell culture apparatus comprising:
a substrate defining a well, wherein the well comprises an interior surface having a hemispheric shape, an exterior surface having an aspheric a convex shape, an upper aperture and a nadir;
wherein light passes between the interior surface and the exterior surface;
wherein the substrate defines a thickness between the interior surface and the exterior surface;
wherein the thickness increases continuously from proximate the upper aperture to the nadir; and
wherein a majority of the well is fully illuminated when exposed to illumination with a brightfield microscope.