	US 12,202,870 B2
	Antigen binding molecules comprising a trimeric TNF family ligand and encoding polynucleotides thereof
Maria Amann, Duebendorf (CH); Peter Bruenker, Hittnau (CH); Christina Claus, Ennetbaden (CH); Claudia Ferrara Koller, Zug (CH); Sandra Grau-Richards, Birmsdorf (CH); Christian Klein, Bonstetten (CH); Viktor Levitski, Schlieren (CH); Ekkehard Moessner, Kreuzlingen (CH); and Pablo Umana, Wollerau (CH)
Assigned to Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed by Hoffmann-La Roche Inc., Little Falls, NJ (US)
Filed on Nov. 18, 2021, as Appl. No. 17/455,617.
Application 17/455,617 is a division of application No. 16/653,652, filed on Oct. 15, 2019, abandoned.
Application 16/653,652 is a division of application No. 15/087,405, filed on Mar. 31, 2016, granted, now 10,464,981, issued on Nov. 5, 2019.
Claims priority of application No. 15161880 (EP), filed on Mar. 31, 2015.
Prior Publication US 2022/0267395 A1, Aug. 25, 2022
Int. Cl. C07K 14/525 (2006.01); A61K 38/00 (2006.01); A61K 39/395 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); C07K 16/40 (2006.01)

CPC C07K 14/525 (2013.01) [A61K 39/395 (2013.01); C07K 14/705 (2013.01); C07K 14/70596 (2013.01); C07K 16/2803 (2013.01); C07K 16/2863 (2013.01); C07K 16/2887 (2013.01); C07K 16/3007 (2013.01); C07K 16/3053 (2013.01); C07K 16/40 (2013.01); A61K 38/00 (2013.01); C07K 2317/31 (2013.01); C07K 2317/526 (2013.01); C07K 2317/55 (2013.01); C07K 2317/60 (2013.01); C07K 2317/622 (2013.01); C07K 2319/035 (2013.01); C07K 2319/30 (2013.01)]

40 Claims

1. A TNF family ligand trimer-containing antigen binding molecule comprising

(a) at least one moiety capable of specific binding to a target cell antigen,

(b) a polypeptide comprising three ectodomains of a TNF ligand family member or fragments thereof that are connected to each other, optionally by peptide linkers, and

(c) a Fc domain composed of a first and a second subunit capable of stable association with each other, wherein the Fc domain is an IgG1 Fc domain or an IgG4 Fc domain, and

wherein the antigen binding molecule comprises

(i) a first heavy chain comprising the VH domain of a Fab molecule capable of specific binding to a target cell antigen,

(ii) a light chain comprising the VL domain of a Fab molecule capable of specific binding to a target cell antigen, and

(iii) a second heavy chain comprising the amino acid sequence of SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO: 8, SEQ ID NO:99, SEQ ID NO: 100, SEQ ID NO: 101 SEQ ID NO:102 or SEQ ID NO: 103.