US 12,201,699 B2
Compositions and methods for promoting homology directed repair
Cecilia Cotta-Ramusino, Cambridge, MA (US)
Assigned to Editas Medicine, Inc., Cambridge, MA (US)
Filed by Editas Medicine, Inc., Cambridge, MA (US)
Filed on Aug. 4, 2021, as Appl. No. 17/393,575.
Application 17/393,575 is a division of application No. 15/518,105, abandoned, previously published as PCT/US2015/055002, filed on Oct. 9, 2015.
Claims priority of provisional application 62/068,371, filed on Oct. 24, 2014.
Claims priority of provisional application 62/062,815, filed on Oct. 10, 2014.
Prior Publication US 2022/0072160 A1, Mar. 10, 2022
Int. Cl. A61K 48/00 (2006.01); C07K 19/00 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/62 (2006.01); C12N 15/64 (2006.01)
CPC A61K 48/0066 (2013.01) [A61K 48/0083 (2013.01); A61K 48/0091 (2013.01); C07K 19/00 (2013.01); C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 15/625 (2013.01); C12N 15/64 (2013.01); C07K 2319/20 (2013.01); C07K 2319/80 (2013.01); C07K 2319/85 (2013.01); C12N 2310/20 (2017.05)] 17 Claims
 
1. A Cas9 system comprising
a Cas9 fusion molecule comprising a Cas9 molecule linked to a template binding domain, and a nucleic acid template system comprising a template binding domain partner and a template nucleic acid,
wherein the template binding domain comprises an affinity for the template binding domain partner and does not bind an endogenous nucleic acid of a cell, wherein the template binding domain partner is exogenous to the cell,
wherein the template binding domain is selected from the group consisting of a TetR repressor protein, or a fragment thereof; a LacI repressor protein, or a fragment thereof; a Gal4 repressor protein, or a fragment thereof; and C1 repressor protein, or a fragment thereof; and
wherein the template binding domain partner is a DNA sequence recognized by a DNA binding protein selected from the group consisting of a Tet-O sequence, a Lac operon O1 sequence, a UAS sequence, and an Operator L and R sequence.