	US 12,201,680 B2
	Nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
Andreas Thess, Kusterdingen (DE); Thomas Schlake, Gundelfingen (DE); and Jochen Probst, Wolfschlugen (DE)
Assigned to CureVac SE, Tübingen (DE)
Filed by CureVac SE, Tübingen (DE)
Filed on Dec. 31, 2020, as Appl. No. 17/139,778.
Application 17/139,778 is a division of application No. 16/938,136, filed on Jul. 24, 2020, granted, now 10,912,826, issued on Feb. 9, 2021.
Application 16/938,136 is a continuation of application No. 15/892,330, filed on Feb. 8, 2018, granted, now 10,799,577, issued on Oct. 13, 2020.
Application 15/892,330 is a continuation of application No. 15/465,322, filed on Mar. 21, 2017, granted, now 10,166,283, issued on Jan. 1, 2019.
Application 15/465,322 is a continuation of application No. 14/378,538, granted, now 9,669,089, issued on Jun. 6, 2017, previously published as PCT/EP2013/000460, filed on Feb. 15, 2013.
Application 14/378,538 is a continuation of application No. PCT/EP2012/000673, filed on Feb. 15, 2012.
Prior Publication US 2021/0128716 A1, May 6, 2021
Int. Cl. A61K 39/145 (2006.01); A61K 39/00 (2006.01); A61K 39/12 (2006.01); C12N 7/00 (2006.01); C12N 15/63 (2006.01); C12N 15/67 (2006.01)

CPC A61K 39/145 (2013.01) [A61K 39/00 (2013.01); A61K 39/12 (2013.01); C12N 7/00 (2013.01); C12N 15/63 (2013.01); C12N 15/67 (2013.01); A61K 2039/53 (2013.01); A61K 2039/64 (2013.01); C12N 2760/16134 (2013.01); C12N 2770/20034 (2013.01); C12N 2830/50 (2013.01); Y02A 50/30 (2018.01)]

23 Claims

1. A method of stimulating an immune response in a subject comprising administering an effective amount a composition to the subject, said composition comprising a mRNA encoding a Severe Acute Respiratory Syndrome (SARS) coronavirus spike(S) protein coding sequence and a histone stem loop sequence, said mRNA provided in complex with a lipid.